In this study, we found there is late ovule initiation at floral stage 10 in the wild-type Arabidopsis when the existing ovules initiated at floral stage 9 start to differentiate, which contributes to around 10% extra ovule number. We previously reported the asynchronous ovule initiation and the hormones integration in regulating ovule initiation. Although the process and its related genes have been reported, the underling mechanisms remains elusive. Understanding the process and regulation of ovule initiation has both scientific significance and potential application. Ovule initiation determines maximum ovule number and has great impact on seed number and yield. Our results give important clues for increasing seed number and yield of cruciferous and leguminous crops. Collectively, this study demonstrates that PIN3 promotes the late ovule initiation and contributes to the extra ovule number. Furthermore, brassinosteroid influences the late ovule initiation through positively regulating PIN3 expression. ![]() Meanwhile, overexpressing STK rescues pin3 phenotypes, suggesting STK participates in PIN3-mediated late ovule initiation possibly by promoting pistil growth. qRT-PCR results indicated that the expression of SEEDSTICK ( STK) is up-regulated under auxin analogues treatment while is down-regulated in pin3 mutants. Physiological analysis illustrated that, unlike picloram, exogenous application of NAA can’t restore these defective phenotypes, implying that PIN3-mediated polar auxin transport is required for the late ovule initiation and pistil length. Genetic analysis revealed that the absence of PIN3 function leads to the reduction in pistil size and the lack of late-initiated ovules, suggesting PIN3 promotes the late ovule initiation process and pistil growth. Here, we further demonstrate that a small amount of ovule primordia initiate at floral stage 10 when the existing ovules initiated at floral stage 9 start to differentiate. We previously reported that most of the ovule primordia initiate asynchronously at floral stage 9 and PINFORMED1 (PIN1) polarization and auxin distribution contributed to this process. However, the regulation of ovule initiation remains largely elusive. 43 members, 159 guests and 36 web crawlers are browsing this forum.Ovule initiation determines the maximum ovule number and has great impact on seed number and yield. Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta, TormatoĢ1,696 topic views. You cannot start new topics / You cannot reply to topics I have Pins! Now Im leaving for a few days! Mushrooms, Mycology and Psychedelics > Mushroom Cultivationĭifferance between hyphal knots and pins? Shop: Buy Kratom Extract, Kratom Powder For Sale Bulk Cannabis Seeds North Spore Mushroom Grow Kits & Cultivation Supplies I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!" Not what is overlay but rather what overlay is ![]() Getting the most out of your casings!, A pinning strategy.
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